PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES ON THE STEM-BARK OF COMMIPHORA MOLLIS (Oliv. ) Engl. (BURSERACAEA)

PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES ON THE STEM-BARK OF COMMIPHORA MOLLIS (Oliv. ) Engl. (BURSERACAEA)

ABSTRACT

Commiphora mollis is traditionally used in the treatment of fever (malaria and typhoid) wound healing, cancer, ulcer and rheumatic condition. The plant grows in Nigeria and across Africa. Phytochemical analysis of methanol extract of the stem-bark of Commiphora mollis showed the presence of flavonoids, saponins, tannins, terpenoids and alkaloids. Extensive Phytochemical studies of ethylacetate water soluble fraction of methanol extract resulted in isolation (55 fractions, 100ml each were collected and pooled together based on similarities in their TLC profile to gave 8 major fractions and repeated gel filtration chromatography of Fraction 5 on Sephadex LH-20 packed column then preparative gave 7.5 mg of compound X1) and characterization using 1HNMR (9 proton signal 5 aromatic proton δ 7.0- 5.94 ppm, 2 oxymithine protons at 4.8 and 4.2 ppm and 2 methylene protons at 2.8 and 2.75 ppm), 13CNMR (15 carbon signals 12 in aromatic region, 2 oxymethine carbon and 1 methylene carbon.), DEPT (7 methine 1 methylene 1 quatenery ), 1H 1H COSY ( correlation between protons at 7.0, H2’ // 6.83, H6’ 7.0, H2’ // 4.8, H2 6.83, H6’ // 4.8, H2 4.8, H2 // 4.2, H3 4.8, H2 // 2.8, H4 4.2 , H3 // 2.8, H4 ), HSQC (correlation between proton and carbon at 7.0, H2’ // 114, C2’ 6.83, H6’ // 118.10, C6’ 6.8, H5’ // 114.60, C5’ 5.8, H6 // 94, C6 4.82, H2 // 78.48, C2 4.21, H3 // 66.10, C3 2.8, H4 and 2.7, H4 // 27.84, C4 ), HMBC and NOESY. The antimicrobial studies of the crude methanol extract, Chloroform and ethylacetate fractions were carried out using disc diffusion and broth agar dilution methods on clinical isolates of Corynebacterium ulcerans, Salmonella typhi, Proteus mirabilis, Bacillus sabtilis, Staphylococcus aureus, Streptococcus pyogens, Escherichia coli, Candida brusei, Shigella dysenteriae and Candida albicans. The crude methanol extract, chloroform and ethylacetate fractions showed strong inhibitory activity against all tested microoganisms with exception of Corynebacterium ulcerans, Salmonella typhi and Proteus mirabilis. Crude methanol extract was found to have MIC at 10 mg/ml for all organisms and vi variable value of MBC/MFC and that of chloroform and ethylacetate fractions have variable value for both MIC and MBC. The stem-bark of Commiphora mollis was rich in bioactive phytochemicals which have antimicrobial activity and could serve as a potential source of compounds effective against disease causing micro- organisms.

BUY THIS TOPIC ON WHATSAPP

Related Project Materials

Background to the study

Democracy is a process of self-government in which citizens operate in their environment directl...

BACKGROUND OF THE STUDY

Agricultural Science is a science subject taught in Nigerian secondary schools...

ABSTRACT

The contribution of taxation to any economy globally cannot be overemphasized. Apart from the...

ABSTRACT

In Nigeria, in spite of the statutory mechanisms put in place to mitigate disputes, the phenomenon has been on the increase and...

ABSTRACT

This project is aimed at producing black and brown polish of good quality.  For clearity, pol...